nature-biotech-cover.gif

Publications

Updated publications from the Chen Lab at the Broad Institute.

Selected Publications

Cable D.M., Murray M., Shanmugam V., Zhang S., Diao M., Chen H., Macosko E.Z., Irizarry R.A. *, †, Chen F. *, † (Nature Methods, 2022) Cell type specific differential expression for spatial transcriptomics. (*,equal contribution; †, Corresponding Author) [Bioarxiv]

Jiang K**., Koob J.**, Chen D.X.**, Krajeski N.R.**, Zhang Y.**, Villger L., Zhou W., Abudayyeh O.O. *, †, Chen F. *, †, Gootenberg J.S. *, †, Programmable eukaryotic protein expression with RNA sensors (*, **equal contribution; †, Corresponding Author) [Bioarxiv]

Preprints


Zhao T.*, Chiang Z.D.*, Morriss J.W., LaFave L.M., Murray E.M., Del Priore I., Meli K., Lareau C.A., Nadaf M.N., Li J., Earl A.S., Macosko E.Z.,  Jacks T.,  Buenrostro J.D.**,  Chen F.** (Nature, 2022) Spatial genomics enables multi-modal study of clonal heterogeneity in tissues  (*, equal contribution, **, Corresponding Author) [Publisher link]

2022


Cleary* B., Murray M., Alam S., Sinha A., Habibi E., Simonton S., Bezney J., Marshall J., Lander E.S.*, Chen F.*, Regev A.* (Nature Biotechnology, 2021) Compressed sensing for imaging transcriptomics (*, Corresponding Author) [Publisher link]

Cable D.M., Murray M., Zou L.S., Goeva A., Macosko E.Z., Chen F.*, †, Irizarry R.A.*, † (Nature Biotechnology, 2021) Robust decomposition of cell type mixtures in spatial transcriptomics (*,equal contribution; †, Corresponding Author) [Publisher link]

Rodriques S.G.*, Chen L.M*., Liu S., Zhong E.D., Scherrer J.R., Boyden E.S.*†, Chen F.*† (Nature Biotechnology 2021) RNA timestamps identify the age of single molecules in RNA sequencing (**, equal contribution; †, Corresponding Author) [Publisher link]

Alon, S.*, Goodwin, D.R.*, Sinha A.S.*, Wassie A.T.*, Chen, F* et al, (Science 2021) Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems. (*, equal contributions) [Publisher link]

2021


Andrew C. Payne*,  Zachary D. Chiang*,  Paul L. Reginato*,  Sarah M. Mangiameli, Evan M. Murray, Chun-Chen Yao,  Styliani Markoulaki,  Andrew S. Earl,  Ajay S. Labade,  Rudolf Jaenisch,  George M. Church,  Edward S. Boyden†,  Jason D. Buenrostro†,  Fei Chen† (Science, 2020) In situ genome sequencing resolves DNA sequence and structure in intact biological samples. (*,equal contribution; †, Corresponding Author) [Publisher link]

Marshall J.L.*, Doughty B.R.*, Subramanian V., Wang Q., Chen L.M., Rodriques S.G., Zhang K., Guckelberger P., Fulco C.P., Nasser J., Grinkevich E.J., Noel T., Mangiameli S., Greka A., Lander E.S.**, †, Chen F.**, †, Engreitz J.M.**, † (PNAS, 2020) HyPR-seq: Single-cell quantification of chosen RNAs via hybridization and sequencing of DNA probes. (*, **, equal contribution; †, Corresponding Author) [Publisher link]

Stickels R.R.*, Murray M.*, Kumar P., Li J., Marshall J.L., Di Bella D., Arlotta P., Macosko E.Z.**, †, Chen F.**, † (Nature Biotechnology, 2020) Highly sensitive spatial transcriptomics at near-cellular resolution with Slide-seqV2 (*, **, equal contribution; †, Corresponding Author) [Publisher link]

Chen H., Liu S., Padula S., Griswold K., Lin A., Zhao T., Marshall J.L., Chen F.* (Nature Biotechnology, 2020) Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. (*, Corresponding Author) [Publisher link]

2020


Rodriques S.G.†, Stickels R.R.†, Goeva A., Martin C.A., Murray E., Vanderburg C.R., Welch J., Chen L.M., Chen F.*††, Macosko E.Z.*†† (Science, 2019) Slide-seq: A Scalable Technology for Measuring Genome-Wide Expression at High Spatial Resolution. (†, ††, equal contribution; *, Corresponding Author) [Publisher link]

2019


Asano S.M., Gao. R., Wassie. A.T., Tillberg P.W., Chen F., Boyden E.S. Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues. Current Protocols in Cell Biology. [Publisher link]

2018


2017

Zhao Y., Bucur O., Irshad H., Chen F., Weins A., Stancu A.L., Oh E-Y., DiStasio M., Torous V., Glass B., Stillman I.E., Schnitt S.J., Beck A.H., Boyden E.S. Nanoscale Imaging of Clinical Specimens Using Pathology-Optimized Expansion Microscopy. Nature Biotechnology. [Publisher link] [PDF]

Chang J.B., Chen F., Yoon Y.G., Jung E. E., Babcock H., Kang J.S., Asano S., Suk H.J., Pak N., Tillberg P.W., Wassie A., Cai D., Boyden E.S. Iterative expansion microscopy. Nature Methods. [Publisher link] [PDF]


2016

Chen F.*, Wassie A.T.*, Cote A.J., Sinha A., Alon S., Asano S., Daugharthy E.R., Chang J.-B., Marblestone A., Church G.M., Raj A., Boyden E.S. Nanoscale Imaging of RNA with Expansion Microscopy. Nature Methods. (*equal contribution) [Publisher link] [PDF]

Tillberg P.W.*, Chen F.*, Piatkevich K.D., Zhao Y., Yu C.-C., English B.P., Gao L., Martorell A., Suk H.-J., Yoshida F., DeGennaro E.M., Roossien D.H., Gong G., Seneviratne U., Tannenbaum S.R., Desimone R., Cai D., Boyden E.S. (2016) Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies. Nature Biotechnology. (*equal contribution) [Publisher link] [PDF]

Zhang Y.S., Chang J.B., Alvarez M.M., Trujillo-de Santiago G., Aleman J., Batzaya B., Kirshnadoss V., Ramanujam A.A., Kazemzadeh-Narbat M., Chen F., Tillberg P.W., Dokmeci M.R., Boyden E.S., Khademhosseini A. Hybrid Microscopy: Enabling Inexpensive High-Performance Imaging through Combined Physical and Optical Magnifications. Scientific Reports. [Publisher link] [PDF]


2015

Chen F.*, Tillberg P.W.*, Boyden E.S. Expansion Microscopy. Science. 347(6221):543-548. (*equal contribution) [Publisher link] [PDF]


2014

Schmidt D., Tillberg P.W.*, Chen F.*, Boyden E.S. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nature Communications. (*equal contribution)  [Publisher link] [PDF]